Best Methods for Studying Bacterial Resistance

I’ve been diving into the world of bacterial resistance patterns lately and I’m curious about the techniques everyone is using. I recently started using PCR for detecting specific resistance genes, but I’m wondering if anyone has found success with alternative methods like whole-genome sequencing or CRISPR-based approaches. What has worked for you in your research?

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Whole-genome sequencing has been a game-changer for us, especially in tracking resistance patterns over time. It’s powerful but can be pricey and time-consuming initially. Have you considered integrating CRISPR for targeted editing alongside your PCR approach? That combo could yield some interesting insights.

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