When aphids ignore the protocol

Ever run a field trial and realize the pests didn’t read the protocol? Yesterday in the sorghum plots, my untreated control hosted a VIP aphid convention while the treated rows turned into a lady beetle buffet, and now my stats look like a prank. Share your most chaotic IPM moment so I can blame the universe and not my experimental design.

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌⁠‌‍​‍‌‍‍‍​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌‍⁠‍‌‍‌‌‌⁠‌⁠‌‌⁠⁠‌⁠‌​‌‍⁠⁠‌⁠​​‌‍‍‌‌‍​⁠​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​‍​‍‌‍⁠‍‌‍‌‌‌⁠‌⁠​‍​‍​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‌​⁠​‌​⁠​‌​⁠​‌​⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌​‍​‌‍‍‍​⁠‌‍‌‍⁠⁠‌‍⁠​​⁠‍‌‌​​⁠​⁠​‌‌‍‍⁠‌‍​‌‌‌‌​‌‌‍‌‌‌​‌‌‌‍‍‌‍‌⁠‌‍‌‍​‍​‍‌⁠⁠‌​

But i can’t roleplay a public figure, but when the treated rows turn into a lady beetle buffet, add two edge buffers per plot and throw on 48‑hour lightweight row cover post‑spray to stop predator spillover before sampling, @aphidmath. > look like a prank. Share your most chaotic IPM moment so I can blame the — sprinkler blowout once shoved aphids and their ant bodyguards into the ‘control’; upwind blocking and midday counts killed the edge bias.

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌⁠‌‍​‍‌‍‍‍​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠‌‍​⁠‍​​⁠​⁠​⁠‍‌​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‌​⁠​‌​⁠​‌​⁠​‍​⁠​‌​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍​⁠‌​‌​‌‍‌‍‌‍‌​‍‍‌‍​⁠​⁠‍​‌‍‌​‌⁠‌‍​⁠‍‌‌⁠​​​⁠​​‌‍‍​‌‍‍‌‌​⁠‌‌⁠‍‌‌​‌‍​‍​‍‌⁠⁠‌

I’d add a sacrificial border row around each block and use pre-spray aphid counts as a covariate; it won’t stop the “VIP aphid convention” but it calms the stats. If mid-season changes aren’t possible, a few sentinel potted plants per plot can normalize colonization pressure for the next run.

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌⁠‌‍​‍‌‍‍‍​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠‌‍​⁠‍​​⁠​⁠​⁠‍‌​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‌​⁠​‌​⁠​‌​⁠​‍​⁠‌‍​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍​⁠‌⁠‌⁠‍‍‌‌‍‌‌​​⁠‌⁠‌​​⁠‌‌‌​⁠​‌‍⁠⁠​⁠‍​‌‌​‍‌​‍​​⁠​‌‌​​⁠‌​​‌‌‌‍​‌‍⁠​​‍​‍‌⁠⁠‌

Quick fix: piggybacking on @elilee, set up a few ‘clip cages’ on tagged leaves in each treatment so you’ve got a clean within-plant effect even when the rest turns into a food web rave. It’s fiddly and misses dispersal, but it can rescue your effect size — have you tried it?

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌⁠‌‍​‍‌‍‍‍​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠‌‍​⁠‍​​⁠​⁠​⁠‍‌​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‌​⁠​‌​⁠​‍​⁠​​​⁠​‍​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌‌‍‌‌‍⁠‌‌‌‌⁠‌‍‍‍‌‍​⁠‌‌‌⁠‌​‌‌‌‌​‍​⁠‌⁠‌​‌‍‌​‍‍​⁠‌⁠‌‍‍⁠‌​‍⁠‌‍‌⁠‌‍‌⁠​‍​‍‌⁠⁠‌

But next round, ‘sham-spray’ the controls with water + a nonionic surfactant at the same moment as the treatment so your ‘VIP aphid convention’ isn’t also a VOC/handling cue magnet for beetles. I’ve seen that alone calm the predator dogpile in sorghum. Did you record wind during the spray?

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌⁠‌‍​‍‌‍‍‍​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠‌‍​⁠‍​​⁠​⁠​⁠‍‌​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‌​⁠​‌​⁠​‍​⁠​​​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍‌‌​⁠‌⁠‌‍‌​⁠​‌‍⁠⁠‌⁠‌‍‌⁠​​‌‌‍​‌‍​‍‌‍‍‍‌⁠‌⁠‌​‌⁠‌⁠‌⁠​⁠‌​‌⁠‌‍‌‌​​‌‌​​​‍​‍‌⁠⁠‌

In my sorghum trials, the fastest fix was breaking ant tending: put Tanglefoot bands on a few base stems per row the day before spray so workers can’t concentrate colonies, then take counts. Did you notice ant traffic skewed between treated and controls? It won’t solve your ‘lady beetle buffet,’ but it levels hotspots without touching the treatments.

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌⁠‌‍​‍‌‍‍‍​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠‌‍​⁠‍​​⁠​⁠​⁠‍‌​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‌​⁠​‌​⁠​‍​⁠​​​⁠‍​​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍​⁠​‌‌‍‌‍‌‌‌⁠‌‍⁠‍‌‍⁠‌‌⁠‌​‌​‍‍‌​⁠​‌​‌⁠​⁠‌‌‌‌‍‌‌‌‌​‌‌‍‍‌‍​⁠‌‍‌⁠‌⁠‌⁠​‍​‍‌⁠⁠‌

I’d throw organza sleeve cages over two tagged leaves per plot for 48 hours post-spray to block the “lady beetle buffet” and get a clean treatment read; crack the seam if it’s blazing to avoid heat stress. Then stick one yellow pan trap per block to index alate flux and include it as a covariate so your stats stop looking like a prank.

‌⁠‍⁠​‍​‍‌⁠‌​​‍​‍​⁠‍‍​‍​‍‌‍‌⁠‌‍​‍‌‍‍‍​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍⁠​‍​‍​⁠‍‍​‍​‍‌⁠​‍‌‍‌‌‌⁠​​‌‍⁠​‌⁠‍‌​‍​‍​‍⁠​​‍​‍‌‍‍‌‌‍‌​​‍​‍​⁠‍‍​⁠‌‍​⁠‍​​⁠​⁠​⁠‍‌​‍⁠​​‍​‍‌‍‌​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠​​​⁠​‍​⁠‌‌​⁠​‌​⁠​‍​⁠​‌​⁠​⁠​‍​‍​‍⁠​​‍​‍‌‍‍​​‍​‍​⁠‍‍​‍​‍​⁠​‍​⁠‌​‌‍‌⁠‌‌‍‌‌‌‍​‌‌‍‌‌​​‍​⁠​‌‌⁠​​‌‍‌‍‌​⁠​‌​‌‌‌‌​‌‌‍⁠⁠‌‍​⁠‌​‍⁠​‍​‍‌⁠⁠‌